Measurements of Alzheimer's Biomarkers

CSF and blood samples from each participant were collected at a single session at approximately 8 am following overnight fasting as previously described.^{5 (link),30 (link)} Concentrations of CSF Aβ40, Aβ42, t-tau, and p-tau181 were measured by chemiluminescent enzyme immunoassay using a fully automated platform (LUMIPULSE G1200; Fujirebio). CSF NfL was measured via commercial ELISA kit (UMAN Diagnostics). Plasma Aβ42 and Aβ40 were measured in the C2N Diagnostics commercial laboratory with the PrecivityAD immunoprecipitation–mass spectrometry assay.^{16 (link)} Plasma p-tau181 and p-tau231 were measured in the Clinical Neurochemistry Laboratory, University of Gothenburg, using in-house Single molecule array (Simoa) assays on an HD-X analyzer (Quanterix), as previously described.^{19 (link),23 (link)} Plasma NfL was measured with Quanterix Nf-Light assay kits at Washington University on a HD-X analyzer. All assays were performed by personnel who were blind to participant information.

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Schindler S.E., Karikari T.K., Ashton N.J., Henson R.L., Yarasheski K.E., West T., Meyer M.R., Kirmess K.M., Li Y., Saef B., Moulder K.L., Bradford D., Fagan A.M., Gordon B.A., Benzinger T.L., Balls-Berry J., Bateman R.J., Xiong C., Zetterberg H., Blennow K, & Morris J.C. (2022). Effect of Race on Prediction of Brain Amyloidosis by Plasma Aβ42/Aβ40, Phosphorylated Tau, and Neurofilament Light. Neurology, 99(3), e245-e257.

Publication 2022

LUMIPULSE G1200 HD-X analyzer Nf-Light assay kits

Assays Blind Blood Clinical laboratory Diagnostics Elisa Enzyme immunoassay Immunoprecipitation Light Mass spectrometry Plasma

Corresponding Organization :

Other organizations : Hope Center for Neurological Disorders, Mallinckrodt (United States), C2N Diagnostics (United States), University College London, UK Dementia Research Institute, University of Pittsburgh, Centre for Mental Health, University of Gothenburg, King's College London, Institute of Neurobiology, National Hospital for Neurology and Neurosurgery

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

CSF Aβ40
CSF Aβ42
CSF t-tau
CSF p-tau181
CSF NfL
Plasma Aβ42
Plasma Aβ40
Plasma p-tau181
Plasma p-tau231
Plasma NfL

control variables

Participants were required to fast overnight prior to sample collection

controls

None explicitly mentioned

Annotations

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