FAST DiI (Invitrogen, D7756) tracing of commissural axons in the spinal cord was performed as previously reported [65 (link)]. FAST DiI (Invitrogen, D7756) labeling of cerebellar parallel fibers was performed as previously described [69 (link)]. DiI (Invitrogen, D282) tracing of optic nerves was performed as previously reported [70 (link)].
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