Malaria Protein Reagents and Assays

WR99210 was kindly provided by Jacobus Pharmaceuticals (Plainsborough Township, NJ). The PKG inhibitor compound 2 (4-[7-[(dimethylamino)methyl]-2-(4-fluorphenyl)imidazo[1,2-α]pyridine-3-yl]pyrimidin-2-amine) was synthesized by LifeArc (London, United Kingdom). Rapamycin, calcium ionophore A23187, the cysteine protease inhibitor E64, and the PDE inhibitor zaprinast were all purchased from Sigma-Aldrich (St. Louis, MO). The calcium chelator Fluo-4-AM was purchased from Thermo Fisher Scientific (Waltham, MA). cGMP, PET-cGMP, and 1-NH₂-cGMP were purchased from the Biolog Life Science Institute (Bremen, Germany).
Rat monoclonal anti-HA tag antibody (clone 3F10) was purchased from Roche LifeScience (Penzberg, Germany) and rabbit anti-human PKG antibody from Enzo Life Sciences (Farmingdale, NY). A rabbit polyclonal antibody against MSP1-30 (53 (link)) as well as a rabbit anti-AMA1 antibody raised against the ectodomain (54 (link)) and rabbit anti-SERA5 (55 (link)) were all described previously. A mouse monoclonal antibody to plasmepsin V was kindly provided by Daniel Goldberg (Washington University School of Medicine in St. Louis, MO).