Adult male transgenic mice expressing a dominant-negative mutation in human PPARγ under the control of an endothelial-specific vascular cadherin promoter (E-V290M) was used as experimental models as reported.17 (link) Age-and sex-matched non-transgenic (NT) littermates were used as controls. A separate study is currently ongoing specifically studying endothelial function in female E-V290M mice and in male and female offspring from E-V290M and NT mice undergoing arginine vasopressin-induced preeclampsia. NT and E-V290M mice were either fed TD.08290 (0.01–0.02% Na, Teklad) as LSD or standard chow (0.3% Na, NIH-31, Teklad) as normal salt diet. There was no difference in body or organ weights in response to diet or genotype (Table S1). Care of the mice met the standards set forth by the National Institutes of Health (NIH) guidelines for the care and use of experimental animals. All procedures were approved by The University of Iowa Animal Care and Use Committee.