Specific pathogen free 4 week old male BALB/c mice were purchased from an animal supplier (Orient Bio, Seongnam, Korea). Mice were housed in laminar flow cages and were maintained on irradiated food and autoclaved water. Mice were acclimatized for 4~5 days before initiation of experimentation. All of our animal maintenance and experimental procedures were approved by Catholic University of Daegu’s IACUC (IACUC-2010-33). The detailed method for induction of atopic dermatitis like immune alterations is described at our previous reports (10 ,11 ,13 (link)). Briefly, BALB/c mice were sensitized twice with 100 mL of 1% DNCB (Sigma, Saint Louis, MO, USA) or vehicle (acetone : olive oil = 4 : 1 mixture; AOO) in a week and challenged twice with 100 L of 0.2% DNCB or the vehicle at the following week on dorsal skin shaved (2 × 4 cm). Mice were sacrificed at 4 days after the second DNCB or vehicle challenge, followed by collection of skin tissues. Each skin tissue was equally divided into two sections; one for protein homogenization and the other for RNA extraction. Number of skin tissues collected were 45 for the AD group, 21 for the vehicle group, and 10 for the normal mice without any treatment.