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Isolation and Culture of Human PBMCs

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Keating S.T., Groh L., van der Heijden C.D., Rodriguez H., dos Santos J.C., Fanucchi S., Okabe J., Kaipananickal H., van Puffelen J.H., Helder L., Noz M.P., Matzaraki V., Li Y., de Bree L.C., Koeken V.A., Moorlag S.J., Mourits V.P., Domínguez-Andrés J., Oosting M., Bulthuis E.P., Koopman W.J., Mhlanga M., El-Osta A., Joosten L.A., Netea M.G, & Riksen N.P. (2020). The Set7 Lysine Methyltransferase Regulates Plasticity in Oxidative Phosphorylation Necessary for Trained Immunity Induced by β-Glucan. Cell Reports, 31(3), 107548.

Publication 2020

Ficoll-Paque RPMI 1640 Dutch-modified culture medium Glutamax pyruvate Escherichia coli lipopolysaccharide (LPS; serotype 055:B5, Laminarin sinefungin

Cells Cyproheptadine Density gradient centrifugation Escherichia coli Ficoll Gentamicin Healthy volunteers Human Inhibitors Isolation Laminarin Monocytes Pam3cys Percoll Peripheral blood mononuclear cells Pyruvate Serum Sinefungin

Corresponding Organization : Radboud Institute for Molecular Life Sciences

Other organizations : Monash University, Council for Scientific and Industrial Research, University of Cape Town, University of Melbourne, Centre for Individualised Infection Medicine, Helmholtz Centre for Infection Research, Medizinische Hochschule Hannover, University of Southern Denmark, Statens Serum Institut, Odense University Hospital, Chinese University of Hong Kong, Prince of Wales Hospital, Iuliu Hațieganu University of Medicine and Pharmacy, University of Bonn

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