Protocol detail

Western Blot Analysis of Cellular Stress Markers

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Protein extraction and western blot analysis were performed as previously described [52 (link)]. Anti-Beclin-1, anti-cleaved caspase 7, anti-CHOP, anti-GRP78, anti-phospho-H2AX, anti-LC3, anti-PARP, and anti-p62 were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-GRP94 was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Anti-α-tubulin was obtained from Abcam (Eugene, OR, USA).

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Shah S.S., Rodriguez G.A., Musick A., Walters W.M., de Cordoba N., Barbarite E., Marlow M.M., Marples B., Prince J.S., Komotar R.J., Vanni S, & Graham R.M. (2019). Targeting Glioblastoma Stem Cells with 2-Deoxy-D-Glucose (2-DG) Potentiates Radiation-Induced Unfolded Protein Response (UPR). Cancers, 11(2), 159.

Publication 2019

Anti-Beclin-1 anti-cleaved caspase 7 anti-CHOP anti-GRP78 anti-phospho-H2AX anti-LC3 anti-PARP anti-p62 Anti-GRP94 Anti-α-tubulin

Beclin 1 Caspase 7 Chop Grp78 Grp94 Protein Western blot analysis α tubulin

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Protein extraction and western blot analysis

dependent variables

Beclin-1
Cleaved caspase 7
GRP78
Phospho-H2AX
GRP94
α-tubulin

control variables

Control variables not explicitly mentioned.

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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