Basing on LB fermentation medium, single factor at a time was used for optimization. The BcMFAse activity was analyzed after incubating for 48 h. Firstly, the basal medium (0.5% yeast extract and 1% NaCl) were supplemented with 1%-3% tryptone to determine the optimal concentration of tryptone. Secondly, the basal medium (the optimal concentration of tryptone and 1% NaCl) were supplemented with 0.3%-1.5% yeast extract to determine the optimal concentration of yeast extract. Finally, according to previous reports, casein hydrolysate helped improving the production of protein (Pedersen et was considered adding to the fermentation medium. The basal medium (optimal concentration of tryptone, optimal concentration of yeast extract and 1% NaCl) were supplemented with 0.2%-1% casein hydrolysate to determine the optimal concentration of casein hydrolysate.
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Optimizing BcMFAse Production Media
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Other organizations : Guangxi University
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Variable analysis
independent variables
- Tryptone concentration (1%-3%)
- Yeast extract concentration (0.3%-1.5%)
- Casein hydrolysate concentration (0.2%-1%)
- BcMFAse activity
- Basal medium (0.5% yeast extract and 1% NaCl)
- Incubation time (48 h)
- Positive control: Not specified
- Negative control: Not specified
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