Cytotoxicity Assay of SKBHT Compound

Cytotoxicity was determined by using Vybrant® MTT assay kit (Thermo Scientific). Similar to the previous report [20 (link)], RAW 264.7 cells (1 × 10⁶ cells) were treated for 16 h with SKBHT dissolved in PBS, and then metabolically active cells were measured by a plate reader (BioTeK, VT, USA) as instructed by the manufacturer's protocol (Thermo Scientific). Live cells were calculated in a percentage against untreated cells. Each assay was conducted in triplicate samples, and measurement was repeated three times.

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Kim K.H., Ahn S., Won R., Lee J.J., Kim T.H., Kim J.I., Choi J.Y, & Joo M. (2020). Sikyungbanha-Tang Suppressing Acute Lung Injury in Mice Is Related to the Activation of Nrf2 and TNFAIP3. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 8125758.

Publication 2020

Vybrant® MTT assay kit

Assay Cells Cytotoxicity Raw 264 7 cells

Corresponding Organization : Pusan National University Yangsan Hospital

Other organizations : Dongseo University, Kyung Hee University

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Variable analysis

independent variables

Concentration of SKBHT dissolved in PBS

dependent variables

Percentage of metabolically active (live) cells

control variables

Cell line (RAW 264.7 cells)
Incubation time (16 h)
Cell density (1 × 10^6 cells)

controls

Untreated cells (negative control)

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