Immortalized Chicken Liver Cell Line Culture

LMH cells, an immortalized chicken liver cell line, were kindly provided by Dr. Jinhua Liu (CAU, Beijing, China). The LMH cells were cultured in Waymouth MB 752/1 Medium (MACGENE Technology, Beijing, China) supplemented with 1× Penicillin-Streptomycin (MACGENE Technology) and 10% fetal bovine serum (Gibco, Grand Island, NE, USA) in a 5% CO₂ incubator at 37 °C. The cell culture plates were coated with 0.1% gelatin solution (Cat. ES-006-B, Millipore, Billerica, MA, USA) and incubated at 4 °C for 10 min before splitting cells. The FAdV4 HuBWH strain was originally isolated from the liver of a diseased chicken [18 (link)] and was stocked at −80 °C till use.