Quantifying Metabolic and Inflammatory Markers

Plasma insulin (E-EL-M1382c, Elabscience, Wuhan, China), GLP-1 (E-EL-M3012, Elabscience), TNF-α (RK00027, ABclonal, Wuhan, China), IL-6 (RK00008, ABclonal), and IL-4 (RK00036, ABclonal) levels were determined via ELISA according to the manufacture’s protocols. Total cholesterol (A111-1-1), triglyceride (A110-1-1), low-density lipoprotein cholesterol (A113-1-1), and high-density lipoprotein cholesterol (A112-1-1) were measured using relative commercial colorimetric enzymatic assay kits from Najing Jiancheng Bioengineering, as described previously [25 (link)].

Free full text: Click here

Zhou Y., Yuan Z., Wang M., Zhang Z., Tan C., Yu J., Bi Y., Liao X., Zhou X., Ali Sheikh M.S, & Yang D. (2023). Liraglutide Attenuates Aortic Valve Calcification in a High-Cholesterol-Diet-Induced Experimental Calcific Aortic Valve Disease Model in Apolipoprotein E-Deficient Mice. Journal of Cardiovascular Development and Disease, 10(9), 386.

Publication 2023

E-EL-M1382c E-EL-M3012 RK00027 RK00008 RK00036

Cholesterol Colorimetric Elisa Enzymatic assay Glp 1 High density lipoprotein cholesterol Insulin Low density lipoprotein cholesterol Plasma Tnf α Triglyceride

Corresponding Organization : Second Xiangya Hospital of Central South University

Other organizations : Al Jouf University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma insulin (E-EL-M1382c, Elabscience, Wuhan, China)
GLP-1 (E-EL-M3012, Elabscience)
TNF-α (RK00027, ABclonal, Wuhan, China)
IL-6 (RK00008, ABclonal)
IL-4 (RK00036, ABclonal)
Total cholesterol (A111-1-1)
Triglyceride (A110-1-1)
Low-density lipoprotein cholesterol (A113-1-1)
High-density lipoprotein cholesterol (A112-1-1)

control variables

None explicitly mentioned

controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!