Serological Assay for Besnoitia Antibodies

Serial dilutions starting at 1:50 of donkey sera were analyzed for Besnoitia antibodies by IFAT using fluorescein (FITC)-conjugate solution [affinity purified goat anti-horse IgG (H + L) (Jackson ImmunoResearch Laboratories, West Grove, USA; diluted 1:50 in PBS)]. The procedure was performed as previously described [10 (link)]. Sera from infected or non-infected donkeys were used as positive and negative controls, respectively, sampled during a besnoitiosis outbreak in north-eastern Pennsylvania [7 (link)].

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Elsheikha H.M., Schares G., Paraschou G., Sullivan R, & Fox R. (2020). First record of besnoitiosis caused by Besnoitia bennetti in donkeys from the UK. Parasites & Vectors, 13, 279.

Publication 2020

goat anti-horse IgG (H + L)

Anti igg Antibodies Besnoitia Besnoitiosis Dilutions Donkeys Fitc Fluorescein Goat Horse Sera

Corresponding Organization : Friedrich-Loeffler-Institut

Other organizations : West Ridge Veterinary Practice

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Variable analysis

independent variables

Dilution of donkey sera starting at 1:50

dependent variables

Presence of Besnoitia antibodies in donkey sera

control variables

Fluorescein (FITC)-conjugate solution [affinity purified goat anti-horse IgG (H + L)]
IFAT procedure as previously described [10]

controls

Sera from infected donkeys (positive control)
Sera from non-infected donkeys (negative control)

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