Endotoxemic Shock Model in Wistar Rats

Male Wistar Han rats (Charles River, Saint-Germain-Nuelles, France) were housed under standard conditions of temperature (21–24 °C), humidity (40–60%) and a 12 h light/dark cycle with the light period starting at 07:00 a.m. Food and water were available ad libitum. Endotoxemic shock was induced in 28-days-old Wistar Han rats and compared to control rats (injection of NaCl 0.9%—CTRL) as previously described [14 (link)]. One hour after LPS injection, the rats were randomly assigned to no therapy (LPS) or fluidotherapy (NaCl 0.9%, 10 mL/kg—LPS + R) ± NButGT (NButGT, 10 mg/kg—NButGT) to increase O-GlcNAcylation levels (Figure 6).