Bioinformatic analyses were performed as described in the Supplementary Methods in S1 Text. The F11 genome was completely sequenced using a combination of long-read Pacific Biosciences Single Molecule Real-Time (SMRT) and short-read Illumina sequencing as previously described [42 (link),43 (link)]. Methods for DNA manipulation, rapid amplification of cDNA ends (5′ RACE), mutant construction, protein preparation, immunoblotting, whole-cell ELISA and β-galactosidase assays were performed as described in the Supplementary Methods in S1 Text.
Sequencing method (long-read Pacific Biosciences Single Molecule Real-Time (SMRT) and short-read Illumina sequencing)
DNA manipulation
Rapid amplification of cDNA ends (5′ RACE)
Mutant construction
Protein preparation
Immunoblotting
Whole-cell ELISA
β-galactosidase assays
dependent variables
F11 genome sequence
control variables
Not explicitly mentioned
controls
Positive and negative controls for the experiments as described in the Supplementary Methods in S1 Text
Annotations
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