Modulation of Alveolar Epithelial Cells

In vitro, rat type II alveolar epithelial cells line (RLE-6TN) was purchased from ATCC (Manassas, USA), and human lung cancer cell (A549) donated by teacher Shao, who works in the Second Affiliated Hospital of Dalian Medical University, all cells grew in DMEM medium (Hyclone, USA) supplemented with 9% fetal bovine serum (FBS; Gibco, USA), the cells were incubated at 37 °C with 5% CO₂. Cells were incubated with recombinant human TGF-β1 (100–21 C, PeproTech) or SFN (1 μmol/L, Sigma S6317)^{14 (link)} in some experiments.

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Zhang Z., Qu J., Zheng C., Zhang P., Zhou W., Cui W., Mo X., Li L., Xu L, & Gao J. (2018). Nrf2 antioxidant pathway suppresses Numb-mediated epithelial–mesenchymal transition during pulmonary fibrosis. Cell Death & Disease, 9(2), 83.

Publication 2018

RLE-6TN FBS recombinant human TGF-β1

Cells Cells line Human Lung cancer Tgf β1

Corresponding Organization :

Other organizations : Anhui Medical University, First Affiliated Hospital of Anhui Medical University, Dalian Medical University, Second Affiliated Hospital of Dalian Medical University

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Variable analysis

independent variables

Recombinant human TGF-β1 (100–21 C, PeproTech)
SFN (1 μmol/L, Sigma S6317)

dependent variables

Not explicitly mentioned

control variables

Cell type: Rat type II alveolar epithelial cells line (RLE-6TN) and human lung cancer cell (A549)
Cell culture medium: DMEM supplemented with 9% fetal bovine serum (FBS)
Incubation conditions: 37 °C with 5% CO2

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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