Malaria Infection Model in Mice

Example 46

CD-1 mice (n=4 per experimental group; female; 6-7-week-old; 20-24 g, Charles River) were inoculated intravenously with approximately 1×10⁵ P. berghei (ANKA GFP-luc) sporozoites freshly dissected from A. stephensi mosquitoes. Immediately after infection, the mice were treated with single oral doses of Compound; infection was monitored as described for the P. berghei erythrocytic-stage assay. For time-course experiments, the time of compound treatment (single oral dose of 10 mg kg⁻¹) was varied from 5 days before infection to 2 days after infection.