For determination of certain blood cytokine levels, as described before (23 (link)), murine IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17, TNF-α, IFN-γ, and MCP-1 Cytometric Bead Array flex sets (BD Biosciences) were used. Samples were acquired with a BD LSR II Fortessa flow cytometer (BD Biosciences) and data were analyzed with BD Biosciences' FCAP software (V3.0).
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