T cell from donors' peripheral blood was primed with dendritic cells pulsed by antigens acquired from breast cancer cells in the presence of interleukins 7 (IL-7, 1 ng/mL, Invitrogen) and 2 (IL-2, 10 u/mL, Gibco) for seven days as previously reported [37 (link)]. The related cytokines were analyzed by related ELISA assays (Sigma, USA).
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