Immunoblot Assay for Src Signaling

Immunoblots were performed according to standard methods [28 (link)] using rabbit antibody anti-human anti-Src (#2109), anti-Phospho-Src (Tyr527, #2105) corresponding in humans to tyrosine 530 residue, anti-Phospho-Src (Tyr416, #2101) corresponding in humans to tyrosine 419 residue, anti PARP (#9542), anti-EGFR (#4267), and anti-EGFR-vIII (#2232; Cell Signaling Technology Inc., Danvers, MA, USA, Upstate, Lake Placid, NY, USA). Anti-rabbit secondary antibody coupled with horseradish peroxidase (Cell Signaling Technologies, Danvers, MA, USA) was utilized.

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Monteleone L., Marengo B., Musumeci F., Grossi G., Carbone A., Valenti G.E., Domenicotti C, & Schenone S. (2022). Anti-Survival Effect of SI306 and Its Derivatives on Human Glioblastoma Cells. Pharmaceutics, 14(7), 1399.

Publication 2022

anti-Phospho-Src anti PARP anti-EGFR anti-EGFR-vIII Anti-rabbit secondary antibody horseradish peroxidase

Anti antibody Antibody Egfr Horseradish peroxidase Humans Immunoblots Rabbit Tyrosine

Corresponding Organization : University of Pisa

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Variable analysis

independent variables

Anti-Src antibody (#2109)
Anti-Phospho-Src (Tyr527, #2105) antibody
Anti-Phospho-Src (Tyr416, #2101) antibody
Anti-PARP (#9542) antibody
Anti-EGFR (#4267) antibody
Anti-EGFR-vIII (#2232) antibody

dependent variables

Src protein expression
Phosphorylation of Src at Tyr527 residue
Phosphorylation of Src at Tyr416 residue
PARP expression
EGFR expression
EGFR-vIII expression

control variables

Standard immunoblotting methods

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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