Culturing Diverse Cell Lines for Research

HeLa, U2OS, and HEK293T cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin in a humidified incubator containing 5% CO2 at 37 °C. DMEM/Nutrient Mixture F-12 medium (DMEM/F-12) was used for culturing nontransformed RPE cells, as previously described (7 (link)). UWB 1.289 cells and UWB 1.289 cells reconstituted with BRCA1 (gifts of Dr Alessandro Vindigni) were cultured at 37 °C with 5% CO2 in 50% RPMI media, 50% Mammary Epithelial Cell Growth Medium BulletKit (Lonza CC-3150) supplemented with 3% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin.