See Key Resources Table for information on the sources of key reagents used in this study.
p-nitrophenyl phosphate (pNPP) and goat anti-human IgG–HRP antibody were obtained from Sigma-Aldrich, Dorset, UK. The semisynthetic macromolecular conjugate of unfractionated heparin and a human serum albumin, APAC, was from Aplagon Oy, Helsinki, Finland. Blocking medium (2.5% horse serum) and 3,3'-diaminobenzidine tetrahydrochloride (DAB) peroxidase substrate for immunohistochemistry were purchased from Vector Laboratories, Peterborough, UK and 3,3,5,5 tetramethylbenzidine (TMB) was from BD Biosciences, Wokingham, UK. Polyclonal phospho-specific G6b-B antibodies were generated by Biogenes, Berlin, Germany. Phalloidin-Alexa 488 was from Invitrogen Life Technologies, Paisley, UK. All other antibodies and chemicals were either purchased or generated as previously described (Mazharian et al., 2012 (link)).
p-nitrophenyl phosphate (pNPP) and goat anti-human IgG–HRP antibody were obtained from Sigma-Aldrich, Dorset, UK. The semisynthetic macromolecular conjugate of unfractionated heparin and a human serum albumin, APAC, was from Aplagon Oy, Helsinki, Finland. Blocking medium (2.5% horse serum) and 3,3'-diaminobenzidine tetrahydrochloride (DAB) peroxidase substrate for immunohistochemistry were purchased from Vector Laboratories, Peterborough, UK and 3,3,5,5 tetramethylbenzidine (TMB) was from BD Biosciences, Wokingham, UK. Polyclonal phospho-specific G6b-B antibodies were generated by Biogenes, Berlin, Germany. Phalloidin-Alexa 488 was from Invitrogen Life Technologies, Paisley, UK. All other antibodies and chemicals were either purchased or generated as previously described (Mazharian et al., 2012 (link)).
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