Two local varieties of Tibetan hulless barley, XQ754 and Nimubai (used and known as tribute barley), were conserved by the Tibet Academy of Agricultural and Animal Husbandry Sciences. Nimubai has a higher amylose content (33.9%) and β-glucan content (7.5%) as compared to XQ754, which had 27.2% amylose and 6.0% β-glucan (data collected from 2009–2010 in Chengdu). The hulless barley plants were cultivated in October, 2010 and grown under normal conditions in the three fields in Chengdu, Sichuan Province of China.
Grains of Nimubai and XQ754 plants were sampled at 5, 10, 15, 20, and 25 days after pollination (dap) for RNA extraction. Each sample consisted of grains from nine individuals. Total RNA was extracted from the grains using Trizol Reagent (Takara) and Fruit-mate for RNA purification (Takara), according to the manufacturer's instructions. The concentration and quality of RNA samples were determined using a Nano Drop 2000 micro-volume spectrophotometer (Thermo Scientific, Waltham, MA, USA). Equal amounts of RNA from each sample of the identical accessions were pooled to construct two cDNA libraries [26] (link), [27] (link).
Grains of Nimubai and XQ754 plants were sampled at 5, 10, 15, 20, and 25 days after pollination (dap) for RNA extraction. Each sample consisted of grains from nine individuals. Total RNA was extracted from the grains using Trizol Reagent (Takara) and Fruit-mate for RNA purification (Takara), according to the manufacturer's instructions. The concentration and quality of RNA samples were determined using a Nano Drop 2000 micro-volume spectrophotometer (Thermo Scientific, Waltham, MA, USA). Equal amounts of RNA from each sample of the identical accessions were pooled to construct two cDNA libraries [26] (link), [27] (link).
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