Sepsis-related miR-451a Expression in PBMCs

The peripheral blood monocyte cells (PBMCs) were isolated and proposed for miR-451a measurement using reverse transcription quantitative polymerase chain reaction (RT-qPCR) in 117 patients with sepsis and 50 HCs. Briefly, the total RNA extraction was accomplished using a PureZOL RNA isolation reagent (Bio-Rad, Hercules, California, United States), and then reverse transcription was performed by the Script™ cDNA Synthesis Kit (Bio-Rad, Hercules, California, United States). Consequently, qPCR reaction was performed by the TB Green™ Fast qPCR Mix (Takara, Kusatsu, Shiga, Japan). The relative expression of miR-451a was calculated based on the 2^–ΔΔCt method using U6 as an internal reference. The detailed primers were designed in line with a previous study (Wang et al., 2020 (link)).