U87-MG, LN-229, and HT-29 cells were obtained from America Type Culture Collection, (ATCC #HTB-14, CRL-2611, and HTB-38, respectively). IGROV-1 and HEY-A8 were obtained from the NCI-60 panel of human cancer cell lines [43 (link)]. U87-MG, LN-229, HT-29, and HEY-A8 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum (FBS; HyClone), 100 U/ml penicillin and 100 μg/ml streptomycin (referred to as 1% Pen/Strep; Life Technologies). IGROV-1 cells were cultured in RPMI-1640 (Life Technologies), supplemented with 2mM L-Glutamine (Life Technologies), 10% FBS and 1% Pen/Strep. Generation and characterization of JHH-136 and JHH-520 cell lines has been previously described [28 (link)]. Both cell lines were grown in suspension in Neurocult NS-A Stem Cell media (StemCell Technology), containing 20 ng/ml human EGF (Peprotech), 10 ng/ml human basic FGF (Peprotech) and 0.2% heparin (StemCell Technology). TSCER2 cells, derived from the human lymphoblast cell line TK6 [44 (link)], were cultured in RPMI 1640 medium (Life Technologies) supplemented with 5% FBS (Gemini Bio-Products), 1 mM sodium pyruvate (Life Technologies) and 1% Pen/Strep.
All cultures were maintained in a 37°C incubator with 5% CO2 and under a humidified atmosphere.
All cultures were maintained in a 37°C incubator with 5% CO2 and under a humidified atmosphere.
Free full text: Click here
