Protocol detail

Comprehensive Protein Expression Analysis

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Western blotting was performed as described previously [38 (link)]. Cell lysates were subjected to SDS–polyacrylamide gel and immunoblot analysis with antibodies against the following proteins: KRAS and HMGA2 (GeneTex); Vimentin (R & D); Cyclin D1 (Millipore); LOX (Abcam); p-FAK (Invitrogen); E-Cadherin, N-Cadherin and b-catenin (BD); MTA1, STAT3, ERK, p-ERK, MEK and p-MEK (Cell Signaling); b-actin (Sigma Aldrich).

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Fan C., Lin Y., Mao Y., Huang Z., Liu A.Y., Ma H., Yu D., Maitikabili A., Xiao H., Zhang C., Liu F., Luo Q, & Ouyang G. (2016). MicroRNA-543 suppresses colorectal cancer growth and metastasis by targeting KRAS, MTA1 and HMGA2. Oncotarget, 7(16), 21825-21839.

Publication 2016

Vimentin Cyclin D1 p-FAK b-actin

Actin Antibodies Catenin Cell Cyclin d1 E cadherin Hmga2 Immunoblot analysis Kras Mta1 N cadherin Polyacrylamide gel Proteins Stat3 Vimentin

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Variable analysis

independent variables

Western blotting procedure

dependent variables

Protein expression of KRAS, HMGA2, Vimentin, Cyclin D1, LOX, p-FAK, E-Cadherin, N-Cadherin, β-catenin, MTA1, STAT3, ERK, p-ERK, MEK, and p-MEK

control variables

β-actin

controls

Positive controls: Not explicitly mentioned.
Negative controls: Not explicitly mentioned.

Annotations

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