Evaluating FLT3 Inhibitor Efficacy

MOLM-13 and MV4-11 cells were seeded at 10,000 cells/well in 96-well plates. After culturing overnight, cells were treated with gilteritinib, quizartinib, or midostaurin in the presence or absence of FL at 25 ng/mL for 3 days [21 (link), 26 (link)]. FLT3^wt- or FLT3-ITD-expressing Ba/F3 cells were seeded at 1,000 cells/well in 96-well plates and treated with gilteritinib or quizartinib for 3 days in the presence or absence of FL at 25 ng/mL for 3 days. Cell viability was measured using the CellTiter-Glo 2.0 Assay (Promega). Measurement of luciferase activity was performed using the ARVO X3 (PerkinElmer) or SpectraMax Paradigm (Molecular Devices). Data were analyzed using GraphPad PRISM 7 software (GraphPad). Cell viability was calculated by defining the survival of untreated cells and medium control wells as 100% and 0%, respectively.