DSS-induced alterations in goblet cells, and subsequent depletion in synthesis and secretion of mucin glycoprotein, were analysed by Alcian blue staining (ab150662 Alcian Blue, pH 2.5 (Mucin Stain), Abcam, Australia) following the manufacturer’s instructions. The staining intensity (IOD) was assessed using Image Pro Plus 7.0 (Media Cybernetics, Inc., Rockville, MD, USA) and used for comparison among groups [34 (link)].
Free full text: Click here