All primer sequences are listed in Supplementary table 2 .
For identification of BRAF amplification in A375BMR cell, genomic DNA
was isolated and quantitative PCR was performed with primers amplifying BRAF,
CRAF and LINE. For identification of the MEK1 mutation in 451LuBRcells, genomic DNA was isolated and MEK1 exon 2 was cloned into the TOPO
TA-cloning vector (450071, Invitrogen), which was followed by Sanger sequencing.
The presence of BRAFV600E/DK in Mel888BMR cell was
identified as previously described9 (link).
Cloning of sgRNAs into LentiCRISPRv2 vector was performed as described
(http://www.genome-engineering.org/crispr/ ). Briefly, the
LentiCRISPRv2 plasmid was digested with BsmBI and gel-purified.
DNA oligonucleotides (Invitrogen) were annealed and ligated into the digested
vector. Target sgRNA oligonucleotide sequences are listed inSupplementary table 2 .
The plasmid encoding the MITF-M isoform was previously described16 . Production of lentivirus was performed
as described previously28 (link).
For identification of BRAF amplification in A375BMR cell, genomic DNA
was isolated and quantitative PCR was performed with primers amplifying BRAF,
CRAF and LINE. For identification of the MEK1 mutation in 451LuBRcells, genomic DNA was isolated and MEK1 exon 2 was cloned into the TOPO
TA-cloning vector (450071, Invitrogen), which was followed by Sanger sequencing.
The presence of BRAFV600E/DK in Mel888BMR cell was
identified as previously described9 (link).
Cloning of sgRNAs into LentiCRISPRv2 vector was performed as described
(
LentiCRISPRv2 plasmid was digested with BsmBI and gel-purified.
DNA oligonucleotides (Invitrogen) were annealed and ligated into the digested
vector. Target sgRNA oligonucleotide sequences are listed in
The plasmid encoding the MITF-M isoform was previously described16 . Production of lentivirus was performed
as described previously28 (link).
