Generation and Characterization of Knockout Mice

C57BL/6 mice, lysozyme M-Cre mice, and Cybb^−/− mice were obtained from The Jackson Laboratory. GFP-LC3 transgenic mice (50 (link)), Mcu^fl/fl mice (18 (link)), and Rubcn^−/− mice (51 (link)) have been previously described. Mcu^Δmye mice were generated by crossing Mcu^fl/fl mice with lysozyme M-Cre mice (18 (link)). Mcu^ΔmyeCybb^−/− and Mcu^ΔmyeRubcn^−/− double knockout (DKO) mice were further generated by crossing Mcu^Δmye mice with Cybb^−/− mice and Rubcn^−/− mice, respectively. All mice were housed in specific pathogen-free facilities and all in vivo experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the Institutional Animal Care and Use Committee. The study was approved by the Ethics Committee of The Ohio State University and all procedures were conducted in accordance with the experimental animal guidelines of The Ohio State University.

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Dong H., Zhao B., Chen J., Liu Z., Li X., Li L, & Wen H. (2022). Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome. Proceedings of the National Academy of Sciences of the United States of America, 119(26), e2123247119.

Publication 2022

C57BL/6 mice lysozyme M-Cre mice Cybb−/− mice

C57bl mice Cybb Ethics committee Experimental animal Institutional animal care and use committee Lysozyme Mice Specific pathogen free Transgenic mice

Corresponding Organization :

Other organizations : The Ohio State University, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

Genotype (C57BL/6 mice, lysozyme M-Cre mice, Cybb−/− mice, GFP-LC3 transgenic mice, Mcu fl/fl mice, Rubcn−/− mice, Mcu Δmye mice, Mcu Δmye Cybb−/− mice, Mcu Δmye Rubcn−/− mice)

dependent variables

Not explicitly mentioned

control variables

Mice were housed in specific pathogen-free facilities
In vivo experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the Institutional Animal Care and Use Committee
The study was approved by the Ethics Committee of The Ohio State University and all procedures were conducted in accordance with the experimental animal guidelines of The Ohio State University

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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