Tryptic soy broth (TSB) (Bacto TSB, BD) prepared according to manufacturer’s directions was used as the culture medium for planktonic growth.
Biofilm media was prepared by adding 0.2% agar powder (AlfaAesar) to TSB media prior to sterilization, and 70 ml of molten agar was poured into deep well petri dishes (Fisher), as previously described [64 (link)].. An aliquot of vAh ML09–119 was removed from cryogenic storage and cultured in TSB overnight at 30 °C on an orbital shaker. This culture was then used to prepare planktonic and biofilm cultures, as previously described [64 (link)]. Briefly, 70 ml of fresh TSB media was inoculated with 1 ml of the overnight culture and grown at 30 °C with shaking to mid-log phase. Biofilm agar plates were inoculated from overnight culture by stab inoculation, sealed with parafilm, and incubated at 30 °C for 72 h. Planktonic and biofilm cultures were performed in triplicate.
Biofilm media was prepared by adding 0.2% agar powder (AlfaAesar) to TSB media prior to sterilization, and 70 ml of molten agar was poured into deep well petri dishes (Fisher), as previously described [64 (link)].. An aliquot of vAh ML09–119 was removed from cryogenic storage and cultured in TSB overnight at 30 °C on an orbital shaker. This culture was then used to prepare planktonic and biofilm cultures, as previously described [64 (link)]. Briefly, 70 ml of fresh TSB media was inoculated with 1 ml of the overnight culture and grown at 30 °C with shaking to mid-log phase. Biofilm agar plates were inoculated from overnight culture by stab inoculation, sealed with parafilm, and incubated at 30 °C for 72 h. Planktonic and biofilm cultures were performed in triplicate.
Free full text: Click here
