ChIP-qPCR was performed as described64 (link), using αCDC73, αLEO1 (Bethyl, Montgomery, TX), αMLLc (gift from Dr. Yali Dou), αFlag (Sigma), αH3K4me3, αH3 (Abcam, Cambridge, UK), IgG (Santa Cruz, Dallas, TX), Stat5-Y694 and Myc-Tag-71D10 (Cell Signaling, Danvers, MA). qPCR primer sequences are listed (Table S1 ).
Protocol detail
ChIP-qPCR Protocol for Chromatin Analysis
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Chip
Primer
Stat5
Corresponding Organization :
Other organizations : University of Michigan–Ann Arbor
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Variable analysis
independent variables
- αCDC73
- αLEO1
- αMLLc
- αFlag
- αH3K4me3
- Stat5-Y694
- Myc-Tag-71D10
- Gene expression (measured by qPCR)
- ChIP-qPCR protocol
- Positive controls: IgG
- Negative controls: Not explicitly mentioned
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