Western blot analysis was performed as previously described [22 (link)]. The following antibodies have been used: p16INK4a (BD Biosciences, Milan, Italy); β-actin (Sigma-Aldrich, St Louis, MO, USA); anti-Pan-Ras (Calbiochem, San Diego, CA, USA); anti IL-8 (ab18672). Immunoreactive bands were visualized using horseradish peroxidase-conjugated secondary antibodies followed by enhanced chemiluminescence (GE Healthcare, Buckinghamshire, UK).
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