Mitochondrial Iron Content Quantification

Isolated mitochondria collected using the Mitochondrial Isolation Kit for Tissue (Pierce, Rockford, IL) were diluted with EDTA-free buffer after sonication. Mitochondrial non-heme iron contents were measured with a commercial Iron Assay Kit (BioAssay Systems, Hayward, CA), which directly detects total iron in the sample, according to the manufacturer’s protocol^{16 (link)}. Isolated mitochondria were sonicated and diluted with EDTA-free buffer, and then iron concentrations were normalized to the mitochondria concentration of each sample.

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Furihata T., Takada S., Kakutani N., Maekawa S., Tsuda M., Matsumoto J., Mizushima W., Fukushima A., Yokota T., Enzan N., Matsushima S., Handa H., Fumoto Y., Nio-Kobayashi J., Iwanaga T., Tanaka S., Tsutsui H., Sabe H, & Kinugawa S. (2021). Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Communications Biology, 4, 138.

Publication 2021

Iron Assay Kit

Bioassay Buffer Edta Heme Iron Mitochondria Mitochondrial Tissue

Corresponding Organization :

Other organizations : Hokkaido University, Kyushu University

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Variable analysis

independent variables

Use of Mitochondrial Isolation Kit for Tissue (Pierce, Rockford, IL)

dependent variables

Mitochondrial non-heme iron contents

control variables

Sonication of isolated mitochondria
Dilution of isolated mitochondria with EDTA-free buffer

controls

None explicitly mentioned

Annotations

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