After the last wash, 1 ml of TRIzol was added to each sample and RNA was extracted similar to as described previously [27 (link)]. mRNA libraries were prepared by the UCSD IGM Genomics Center using Illumina Stranded mRNA Prep kit and sequenced on a single lane of an Illumina HiSeq 4000, using 100 bp paired-end reads.
Free full text: Click here