Osteogenic differentiation induction was performed on hPDLSCs. When the cell confluence reached about 70%, mineralization induction solution (α-MEM medium containing 10% FBS, 1% PS, 50 ng/mL ascorbic acid, 10 mmol/mL β-glycerophosphate sodium and 4 ng/mL dexamethasone) was added. And the solution was changed every 3 d, ALP staining was performed until the 4th day of culture, and alizarin red staining was conducted on the 14th day to observe the osteogenic differentiation.
Adipogenic differentiation induction was performed on hPDLSCs. After the cells were completely grown, adipogenic induction solution A (α-MEM medium containing 10% FBS, 1% PS, 10 μg/mL insulin, 1 μmol/L indomethacin, and 0.5 mmol/L IBMX) was added. After 3 d, B solution (α-MEM medium containing 10% FBS, 1% PS, and 10 μg/mL insulin) took the place of solution A. One day later, solution A substituted for solution B, while 3 d later, solution B again replaced A. Eventually, oil red O staining was utilized 1 d later for adipogenesis detection.
Adipogenic differentiation induction was performed on hPDLSCs. After the cells were completely grown, adipogenic induction solution A (α-MEM medium containing 10% FBS, 1% PS, 10 μg/mL insulin, 1 μmol/L indomethacin, and 0.5 mmol/L IBMX) was added. After 3 d, B solution (α-MEM medium containing 10% FBS, 1% PS, and 10 μg/mL insulin) took the place of solution A. One day later, solution A substituted for solution B, while 3 d later, solution B again replaced A. Eventually, oil red O staining was utilized 1 d later for adipogenesis detection.
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