Cells were sonicated in 0.3 mL of PBS containing internal standards (100 ng of margaric acid). Fatty acids were extracted using an ISOLUTE SLE + column and dichloromethane. The organic fractions were dried under a nitrogen stream. The residue was dissolved in 5 µL of pyridine and 30 µL of BSTFA + TMCS (99:1) (TS-38831; Thermo Fisher Scientific, Waltham, MA, USA) for trimethylsilylation. The derivatization reaction was performed for 30 min at 40 °C7 .
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Fatty Acid Extraction and Derivatization
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Other organizations : Nihon University, Aichi Medical University, Keio University
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Variable analysis
independent variables
- Sonication in 0.3 mL of PBS containing internal standards (100 ng of margaric acid)
- Fatty acid composition
- Internal standard (100 ng of margaric acid)
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