Ginsenoside Profiling by Mass Spectrometry

Data acquisition was performed on Thermo Xcalibur software (version 4.5) whereas data processing and data mining were performed on Free Style^TM 1.8 SP1 software and Compound Discoverer^TM software (Thermo Scientific^TM, version 3.2.0.421). Negative-ion mode was adopted to perform data acquisition due to ginsenosides exhibited high [M – H]^– and/or [M – H + HAc]^– ion responses in a full scan in this mode. The extracted solution of RRPG was first analyzed in a full scan mode so as to minimize the losses of signals and then targeted MS/MS analysis was followed after constructing the precursor ion lists.

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Wang H.P., Wang Z.J., Du J., Lin Z.Z., Zhao C., Zhang R., Yin Q., Fan C.L., Peng P, & Wang Z.B. (2023). Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of Panax ginseng Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis. Molecules, 28(3), 941.

Publication 2023

Xcalibur software Compound DiscovererTM

Ginsenosides Ms ms Scan

Corresponding Organization : Beijing Tongren Hospital

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Variable analysis

independent variables

Data acquisition software
Data processing and data mining software

dependent variables

Negative-ion mode
[M - H]- and/or [M - H + HAc]- ion responses in a full scan

control variables

Negative-ion mode for data acquisition
Full scan mode for initial analysis

controls

No positive or negative controls were explicitly mentioned.

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