Evaluating Anti-Inflammatory Effects of Catalpol on Diesel Exhaust Particles-Induced Toxicity

The measurement of cytochrome C (R&D Systems, Minneapolis, MN, USA), cleaved caspase C (R&D Systems, Minneapolis, MN, USA) and phosphorylated NF-κB (Cell Signalling Technology, Danvers, MA, USA) in heart homogenates, obtained from mice i.t. instilled with DEPs or saline with or without catalpol treatment, were obtained using commercially available ELISA kits. Moreover, protein expressions for phosphorylated NF-κB and phosphorylated NF-κB inhibitor α (IκBα) were further measured using the Western blotting technique, as previously reported [20 (link),26 (link),27 (link)].

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Nemmar A., Beegam S., Zaaba N.E., Alblooshi S., Alseiari S, & Ali B.H. (2022). The Salutary Effects of Catalpol on Diesel Exhaust Particles-Induced Thrombogenic Changes and Cardiac Oxidative Stress, Inflammation and Apoptosis. Biomedicines, 10(1), 99.

Publication 2022

cytochrome C phosphorylated NF-κB

Caspase Catalpol Cytochrome c Deps Elisa Heart Mice Nf κb Nf κb inhibitor Protein Saline

Corresponding Organization : United Arab Emirates University

Other organizations : Sultan Qaboos University

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Variable analysis

independent variables

Diesel exhaust particles (DEPs)
Catalpol treatment

dependent variables

Cytochrome C
Cleaved caspase C
Phosphorylated NF-κB
Phosphorylated NF-κB
Phosphorylated NF-κB inhibitor α (IκBα)

control variables

Saline

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