Microdialysis of Arterial Glucose

24 h following recovery, mice were anaesthetized using isoflurane, and another microdialysis probes were implanted into the femoral artery. Then microdialysis probes were continuously perfused with artificial cerebrospinal fluid (aCSF, 147 mM NaCl, 3 mM KCl, 1 mM MgCl₂·6H₂O, 0.787 mM CaCl₂·6H₂O, 186 μM Ascorbate, 3.52 mM NaH₂PO₄·H₂O, pH 7.4) at the work flow-rate of 1 μL/min (CMA/400 microdialysis pump, Solna, Sweden) [43 (link)]. The microdialysis aliquots were collected during the final 60 min of the 2-h study period when the experiment had reached steady-state concentrations of the substrates. Dialysate glucose was measured by the glucose oxidase method.