In this study, the rapeseed genotype “ZS11”, a typical cultivated variety, was used for the stress treatments. The seeds of the ZS11 genotype were furnished by OCRI, CAAS, China. Before the stress treatments, some seeds were randomly selected from the same batch of seeds to determine the germination rate. The seeds with a 100% germination rate were considered to be vigorous seeds. The vigorous seeds were carefully chosen and sterilized with a 10% hypochlorous acid solution for 5 min. The seeds were grown on water-saturated filter paper in a chamber (25 °C day and night, and a 16 h/8 h light/dark cycle) until the plants grew to the five-leaf stage. When the plants had grown to the five-leaf stage, we stopped watering to impose drought stress. Samples from the stem were collected 0, 1, 2, 3, 4, 5, 6 and 7 days after the initiation of the drought treatment [67 (link),68 (link)]. Moreover, three biological replicates of each sample were collected. The gathered stems were immediately put into liquid nitrogen and kept in a freezer for later use at −80 °C.
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