Castration-Sensitive and Castration-Resistant Prostate Cancer Cell Lines

The human PCa cell lines LNCaP and PC3 were obtained from the American Type Culture Collection (ATCC). Prof. Thalmann (University of Berne, Berne, Switzerland) kindly provided the C4-2 cell line [23 (link)]. LNCaP and its subcell line C4-2 represent a castration sensitive (LNCaP) and castration-resistant (C4-2) phenotype. LNCaP, C4-2, and PC3 were cultured as described in Erb et al., 2020 [26 (link)]. The main characteristics of the used cell lines are listed in Table 1. Mycoplasma testing was performed using the Mycoalert Detection Assay (Lonza, Basel, Switzerland). STR profiling was used to verify cell line authentication. For the experiments, steroid hormone and growth factors withdrawal were achieved by a medium change to 5% dextran-coated charcoal treated FBS (FBSdcc; Thermo Fischer Scientific, Waltham, MA, USA). Androgen treatment was performed using the synthetic androgen R1881 (Sigma-Aldrich, St. Louis, MO, USA, R0908-10MG, Lot No: 085M4610V) dissolved in DMSO (20 µM stock solution), and aliquots were stored at −80 °C.