Immunohistological Characterization of Tissue

Immunohistology studies were carried out as described (Pawlisz et al., 2008 (link)) on 12 μm frozen or 5 μm paraffin sections. The following antibodies were used: FLNA, Pals 1, Igf2 (Epitomics); Cux1, DCX (Santa Cruz); Pax6, Neurofilament, ZO1, NaK ATPase (Developmental Study Hybridoma Bank); BrdU B44, βCatenin, E-Cadherin, N-Cadherin, VE-Cadherin, PECAM, Fibronectin, beta Integrin (BD Biosciences); Tuj1, Pericentrin (Covance); BrdU BU1/75, Foxp1, Foxp2, SATB2, Ctip2, Glast, (Abcam, Cambridge, MA); GFAP (Dako); NeuN, Tbr2, Tbr1, HABP (Millipore); panCadherin, GFP (Life Technologies); MAP2, Biotin-IB4 (Sigma); PDGFRβ (eBioscience); Alexa Fluor 488 Phalloidin and Alexa Fluor 546 Phalloidin and fluorescence conjugated secondary antibodies were from Life Technologies. All experiments were repeated with at least three independent litters and representative images are shown.

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Houlihan S.L., Lanctot A.A., Guo Y, & Feng Y. (2016). Upregulation of neurovascular communication through filamin abrogation promotes ectopic periventricular neurogenesis. eLife, 5, e17823.

Publication 2016

BrdU B44 βCatenin E-Cadherin N-Cadherin VE-Cadherin Fibronectin GFAP PDGFRβ Alexa Fluor 488 Phalloidin Alexa Fluor 546 Phalloidin

Alexa fluor 488 Alexa fluor 546 Antibodies Atpase Biotin Brdu E cadherin Fibronectin Fluorescence Frozen Gfap Hybridoma Igf2 Integrin Litters Map2 N cadherin Neurofilament Pals Paraffin Pdgfrβ Pericentrin Phalloidin Ve cadherin

Corresponding Organization :

Other organizations : Northwestern University

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Variable analysis

independent variables

12 μm frozen sections
5 μm paraffin sections

dependent variables

Immunohistology studies

control variables

Independent experiments with at least three independent litters

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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