Unless otherwise noted, all reagents were purchased from Sigma Aldrich or Fisher Scientific and used without further purification. 5-norbornene-2-carboxylic acid (97 %, predominately endo isomer) was purchased from Alfa Aesar and used without further purification. Dulbecco’s modified Eagle’s media (DMEM), phosphate buffered saline (PBS), fetal bovine serum (FBS), penicillin–streptomycin, Amphotericin B, and trypsin/EDTA were all purchased from ThermoFisher. 8-arm 20 kDa poly(ethylene glycol) thiol was purchased from JenKem Technologies and used without further purification. The norbornene functionalized cell adhesion peptide Norborne-RGDSG was synthesized according to a previously published protocol.16 (link) NIH/3T3 fibroblasts were acquired from the American Type Culture Collection (ATCC CRL1658).
Protocol detail
Photoactive Hydrogel for Cell Culture
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5-norbornene-2-carboxylic acid
penicillin–streptomycin
Amphotericin B
trypsin/EDTA
NIH/3T3 fibroblasts
Amphotericin b
Carboxylic acid
Cell adhesion
Eagle
Edta
Endo
Fetal bovine serum
Fibroblasts
Isomer
Nih 3t3
Norbornene
Penicillin
Peptide
Phosphate
Poly ethylene glycol
Saline
Streptomycin
Thiol
Trypsin
Corresponding Organization :
Other organizations : University of Colorado Boulder
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Variable analysis
independent variables
- Norbornene functionalized cell adhesion peptide Norborne-RGDSG
- 8-arm 20 kDa poly(ethylene glycol) thiol
- Not explicitly mentioned
- 5-norbornene-2-carboxylic acid (97 %, predominately endo isomer)
- Dulbecco's modified Eagle's media (DMEM)
- Phosphate buffered saline (PBS)
- Fetal bovine serum (FBS)
- Penicillin–streptomycin
- Amphotericin B
- Trypsin/EDTA
- NIH/3T3 fibroblasts
- Positive control: Not mentioned
- Negative control: Not mentioned
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