The rates of oxygen consumption (OCR) in various cybrid cell lines were measured with a Seahorse Bioscience XF-96 extracellular flux analyzer (Seahorse Bioscience), as detailed previously (61 (link),62 (link)). Cybrid cells were seeded at a density of 2 × 104 cells per well on Seahorse XF96 polystyrene tissue culture plates (Seahorse Bioscience). Inhibitors were used at the following concentrations: oligomycin (1.5 μM), carbonyl cyanide 4-trifluoromethoxy-phenylhydrazone (FCCP) (0.8 μM), antimycin A (1.5 μM) and rotenone (3 μM).
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Measuring Oxygen Consumption in Cybrid Cells
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Antimycin a
Carbonyl cyanide phenylhydrazone
Cell lines
Cells
Fccp
Inhibitors
Oligomycin
Oxygen consumption
Polystyrene
Rotenone
Seahorse
Tissue
Corresponding Organization :
Other organizations : Zhejiang University, Children's Hospital of Zhejiang University
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Variable analysis
independent variables
- Inhibitor concentrations: oligomycin (1.5 μM), carbonyl cyanide 4-trifluoromethoxy-phenylhydrazone (FCCP) (0.8 μM), antimycin A (1.5 μM) and rotenone (3 μM)
- Rates of oxygen consumption (OCR) in various cybrid cell lines
- Cybrid cells were seeded at a density of 2 × 10^4 cells per well on Seahorse XF96 polystyrene tissue culture plates (Seahorse Bioscience)
- No positive or negative controls were explicitly mentioned in the provided information.
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