NOD mice were originally procured from Taconic Biosciences, Inc. Anti-insulin VDJH-125 from our original H+L chain 125Tg (25 (link)) mice was cloned to generate a targeting vector, pIVH-SAH-125-VDJH (21 (link)). Founder mice were generated by targeting 129Ola embryonic stem cells and then backcrossed to NOD for at least 10 generations. Southern blot was used to confirm the presence of the targeted allele. All mice were housed under specific pathogen-free conditions, and all studies were approved by the institutional use and animal care committee of Vanderbilt University Medical Center, fully accredited by the AAALAC. Both male and female mice were used to characterize B cell development and function while female mice were employed in disease studies.
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Targeted Immunodeficient NOD Mouse Model
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Allele
Animal care committee
B cell
Embryonic stem cells
Female
Insulin
Male
Mice
Nod mice
Southern blot
Specific pathogen free
Vector
Corresponding Organization : Vanderbilt University
Other organizations : Vanderbilt University Medical Center
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Variable analysis
independent variables
- Targeting of anti-insulin VDJH-125 from original H+L chain 125Tg (25 (link)) mice to generate a targeting vector, pIVH-SAH-125-VDJH (21 (link))
- B cell development and function
- Disease studies in female mice
- NOD mice originally procured from Taconic Biosciences, Inc.
- Founder mice generated by targeting 129Ola embryonic stem cells and then backcrossed to NOD for at least 10 generations
- Presence of targeted allele confirmed by Southern blot
- Mice housed under specific pathogen-free conditions
- Studies approved by the institutional use and animal care committee of Vanderbilt University Medical Center, fully accredited by the AAALAC
- Positive control: None specified
- Negative control: None specified
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