The PMA and AgNPs-CIT effects on the viability of the MCF-7 cell-lines were determined invitro. The MCF-7 cell-lines (1 × 106/mL) were placed in 35-mm culture dishes (Becton-Dickinson, Franklin Lakes, NJ, USA) in DMEM medium, or serum-starved for 12 hr/overnight. The MCF-7 cell-lines were cultured without PMA, or AgNPs-CIT served as controls. The cells were treated with various doses of AgNPs-CIT (0.5 to 160 μM) for 24–48 h of the incubation, and the cytotoxicity was examined using the CytoTox-Glo™, Cytotoxicity Assay Kit (Promega, Madison, WI, USA) as described.35 (link) In another set of experiments, MCF-7 cell-lines were pretreated with different doses of AgNPs-CIT (0.5–10 µM) for 2 h before its stimulation with PMA (0.5 µM) according to the reported procedure.36 (link),37 (link)
Protocol detail
Cytotoxicity of AgNPs-CIT on MCF-7 Cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Al-Azhar University
Other organizations : University College Cork, Minia University Hospital
Top 5 similar protocols
Protocol cited in 2 other protocols
Variable analysis
independent variables
- Different doses of AgNPs-CIT (0.5 to 160 μM)
- Different doses of AgNPs-CIT (0.5–10 µM) for 2 h before stimulation with PMA (0.5 µM)
- Cytotoxicity of MCF-7 cell-lines
- MCF-7 cell-lines (1 × 106/mL) were placed in 35-mm culture dishes in DMEM medium, or serum-starved for 12 hr/overnight
- MCF-7 cell-lines were cultured without PMA, or AgNPs-CIT served as controls
- MCF-7 cell-lines treated with PMA (0.5 µM)
- MCF-7 cell-lines cultured without PMA or AgNPs-CIT
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!