Total RNA from adrenal glands of 2-month-old animals was extracted using the Total RNA Mini Kit (FroggaBio) according to the manufacturer's protocol. RNA (n = 3 per group) was submitted to the McGill University and Génome Québec Innovation Centre (Montreal, Quebec, Canada) for RNA quality control and microarray analysis using the Affymetrix Clariom S Mouse assay. Results were analyzed using Transcriptome Analysis Console software (TAC 4.0) (Thermo Fisher Scientific). Thresholds used for P value and fold change were .05 and ±2, respectively. Further analyses were performed using the Metascape gene annotation and analysis resource [18 (link)]. A protein–protein interaction profile was additionally performed using the string database [19 (link)].
Protocol detail
Adrenal Gland Transcriptome Profiling
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
A protein
Adrenal glands
Animals
Assay
Gene annotation
Microarray analysis
Mouse
Transcriptome analysis
Corresponding Organization : Université de Montréal
Top 5 similar protocols
Variable analysis
independent variables
- Age of animals (2-month-old)
- Gene expression profiles measured using microarray analysis
- Adrenal gland tissue samples
- RNA extraction method (Total RNA Mini Kit)
- RNA quality control and microarray analysis (Affymetrix Clariom S Mouse assay)
- Bioinformatics analysis (Transcriptome Analysis Console software and Metascape gene annotation)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!