Cell Colony Formation Assay

Cell colony formation assay was performed as previously described [44 (link)]. Briefly, the treated cells at 1 × 10³ cells/well were suspended in 1 mL of DMEM containing 0.6% low-melting-point agarose (Amresco, USA) and 10% FBS, and plated on a bottom layer containing 0.3% agarose and 10% FBS in 6-well plate in triplicate. The 6-well cell plates were added Ary at 1.25, 2.5, 5, 10 or 20 μg/ mL. After being incubated for two weeks, the cell plates were stained with 0.1% crystal violet for 15 min, and the colonies were counted under a microscopy.

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Ming P., Cai T., Li J., Ning Y., Xie S., Tao T, & Tang F. (2016). A novel arylbenzofuran induces cervical cancer cell apoptosis and G1/S arrest through ERK-mediated Cdk2/cyclin-A signaling pathway. Oncotarget, 7(27), 41843-41856.

Publication 2016

low-melting-point agarose agarose

Agarose Assay Cell Crystal violet Microscopy

Corresponding Organization : Jinan University

Other organizations : Hubei University of Chinese Medicine, Shenzhen Second People's Hospital, Shanghai Institute of Pharmaceutical Industry

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Variable analysis

independent variables

Ary concentration (1.25, 2.5, 5, 10 or 20 μg/mL)

dependent variables

Cell colony formation

control variables

Cell density (1 × 10^3 cells/well)
Culture medium (DMEM containing 0.6% low-melting-point agarose and 10% FBS)
Incubation time (two weeks)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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