Quantification of miR-375 and Genes

The procedure for PCR quantification was previously described (13 (link),18 (link)–20 (link)). The expression levels of miR-375 (assay ID: 000564; Applied Biosystems, Foster City, CA, USA) were analyzed by TaqMan qRT-PCR assays (TaqMan MicroRNA assays; Applied Biosystems) and RNU48 (assay ID: 001006) was used for normalization. TaqMan probes and primers for MMP-13 (assay ID: Hs00233992_m1; Applied Biosystems), CENPF (assay ID: Hs01118845_m1), KIF14 (assay ID: Hs00978236_m1) and GUSB (the internal control; assay ID: Hs00939627_ml; Applied Biosystems) were used for gene expression analysis.

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Osako Y., Seki N., Kita Y., Yonemori K., Koshizuka K., Kurozumi A., Omoto I., Sasaki K., Uchikado Y., Kurahara H., Maemura K, & Natsugoe S. (2016). Regulation of MMP13 by antitumor microRNA-375 markedly inhibits cancer cell migration and invasion in esophageal squamous cell carcinoma. International Journal of Oncology, 49(6), 2255-2264.

Publication 2016

miR-375 TaqMan MicroRNA assays CENPF

Assay Cenpf Gene expression analysis Microrna Mmp 13 Primers

Corresponding Organization :

Other organizations : Kagoshima University, Chiba University

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Variable analysis

independent variables

Expression levels of miR-375

dependent variables

Expression levels of MMP-13
Expression levels of CENPF
Expression levels of KIF14

control variables

RNU48 (used for normalization)
GUSB (the internal control)

controls

Positive control: Not specified
Negative control: Not specified

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