The experimental animals were divided into five groups of eight rats each.
Group I (Control group): control rats received 0.3% sodium carboxymethyl cellulose (CMC-Na) solution (2 ml kg−1 day−1, i.g.) for a period of 21 days and normal saline (1 ml kg−1, s.c.) on 20th and 21st day.
Group II (ISO group): rats received 0.3% CMC-Na solution for a period of 21 days and ISO (85 mg kg−1, s.c.) in normal saline on 20th and 21st day at an interval of 24 h.
Group III (Pae+ISO group): rats received paeonol (80 mg kg−1 day−1, i.g.) for a period of 21 days and ISO on 20th and 21st day.
Group IV (DSS+ISO group): rats received danshensu (160 mg kg−1 day−1, i.g.) for a period of 21 days and ISO on 20th and 21st day.
Group V (Pae+DSS+ISO group): rats received paeonol (80 mg kg−1 day−1, i.g.) and danshensu (160 mg kg−1 day−1, i.g.) for a period of 21 days and ISO on 20th and 21st day.
Paeonol and danshensu were suspended in 0.3% CMC-Na solution. Control and ISO treated group received equal quantity of vehicle.
At the end of the experimental period, rats were anesthetized with pentobarbital sodium (35 mg kg−1, i.p.), needle electrodes were inserted under the skin of the animals in lead II position. Electrocardiograph recordings were made using BL-420S Biologic Function Experiment system (Technology & Market Co., Ltd., Chengdu, China) and ST-segment elevation or depression (expressed in mv) in normal and experiment animals were considered.
After recording the ECG, blood was collected by abdominal aorta and allowed to clot for 1 h at room temperature. Serum was subsequently separated by centrifugation at 3500×g for 15 min and stored at −80°C for biochemical assays. After blood collection, rats were sacrificed by cervical decapitation. Heart tissue was excised immediately and rinsed in ice-cold normal saline, then homogenized by an IKA T10 Basic homogenizer (Staufen, Germany) in 0.05M ice-cold phosphate buffer (pH 7.4, 1∶10 w/v). The homogenate was centrifuged at 12000×g for 10 min at 4°C and the supernatant was stored at −80°C for the estimation of various biochemical parameters.
Group I (Control group): control rats received 0.3% sodium carboxymethyl cellulose (CMC-Na) solution (2 ml kg−1 day−1, i.g.) for a period of 21 days and normal saline (1 ml kg−1, s.c.) on 20th and 21st day.
Group II (ISO group): rats received 0.3% CMC-Na solution for a period of 21 days and ISO (85 mg kg−1, s.c.) in normal saline on 20th and 21st day at an interval of 24 h.
Group III (Pae+ISO group): rats received paeonol (80 mg kg−1 day−1, i.g.) for a period of 21 days and ISO on 20th and 21st day.
Group IV (DSS+ISO group): rats received danshensu (160 mg kg−1 day−1, i.g.) for a period of 21 days and ISO on 20th and 21st day.
Group V (Pae+DSS+ISO group): rats received paeonol (80 mg kg−1 day−1, i.g.) and danshensu (160 mg kg−1 day−1, i.g.) for a period of 21 days and ISO on 20th and 21st day.
Paeonol and danshensu were suspended in 0.3% CMC-Na solution. Control and ISO treated group received equal quantity of vehicle.
At the end of the experimental period, rats were anesthetized with pentobarbital sodium (35 mg kg−1, i.p.), needle electrodes were inserted under the skin of the animals in lead II position. Electrocardiograph recordings were made using BL-420S Biologic Function Experiment system (Technology & Market Co., Ltd., Chengdu, China) and ST-segment elevation or depression (expressed in mv) in normal and experiment animals were considered.
After recording the ECG, blood was collected by abdominal aorta and allowed to clot for 1 h at room temperature. Serum was subsequently separated by centrifugation at 3500×g for 15 min and stored at −80°C for biochemical assays. After blood collection, rats were sacrificed by cervical decapitation. Heart tissue was excised immediately and rinsed in ice-cold normal saline, then homogenized by an IKA T10 Basic homogenizer (Staufen, Germany) in 0.05M ice-cold phosphate buffer (pH 7.4, 1∶10 w/v). The homogenate was centrifuged at 12000×g for 10 min at 4°C and the supernatant was stored at −80°C for the estimation of various biochemical parameters.
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